Fig. 4

The 20E concentration in the salivary gland and hemolymph of Rhipicephalus haemaphysaloides. Tissues were dissected from female ticks. The 20E concentration in a the salivary gland (ANOVA, Engorged-0h vs Fed5, F (7, 8), P = 0.9994; Fed5 vs Engorged-24h, F (7, 8), P < 0.0001; Engorged-24h vs Engorged-48h, F (7, 8), P = 0.0619; Engorged-48h vs Engorged-72h, F (7, 8), P = 0.0289; Engorged-72h vs Engorged-96h, F (7, 8), P = 0.424; Engorged-96h vs Engorged-120h, F (7, 8), P = 0.4278; Engorged-120h vs unfed, F (7, 8), P = 0.0007) and b hemolymph (ANOVA, Engorged-48h vs Engorged-72h, F (7, 8), P = 0.0101; Engorged-72h vs Engorged-24h, F (7, 8), P > 0.9999; Engorged-24h vs Engorged-0h, F (7, 8), P = 0.9455; Engorged-0h vs Fed5, F (7, 8), P = 0.9994; Fed5 vs Engorged-120h, F (7, 8), P = 0.2216; Engorged-120h vs Engorged-96h, F (7, 8), P = 0.9998; Engorged-96h vs unfed, F (7, 8), P < 0.0001), during blood-feeding and the post-engorgement period were measured by EIA (in pg/μg or μl tissue). The data represent the mean ± SD of with 10 mg salivary gland or 30 μl hemolymph at each time point, and samples from different ticks were analyzed in duplicate. One-way ANOVA for statistical differences between multiple groups was used (α = 0.05)