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Table 1 Statistical analysis of biological effects on dsRhEcR-treated and dsRhUSP-treated groups. It showed that the dsRhEcR-treated group had significant inhibition of feeding time, body weight, engorgement rate, oviposition rate, and egg-hatching rate compared to the dsLuciferase-treated group and that the dsRhUSP-treated group did not induce tick alterations

From: The ecdysteroid receptor regulates salivary gland degeneration through apoptosis in Rhipicephalus haemaphysaloides

Group

Attachment rate in 24 h

Feeding period (days)

Engorged body weight (g)

Engorgement rate

Oviposition rate

Egg-hatching rate

dsEcR

50% (40/80)

7-10

0.0448 ± 0.0131*

0

0

0

dsLuciferase

47.5% (38/80)

7-8

0.2834 ± 0.0654

81.25% (65/80)

90% (18/20)

100%

dsUSP

62.5% (50/80)

7-8

0.2938 ± 0.0566**

85% (68/80)

95% (19/20)

100%

  1. Student’s t-test was used to compare the weight of the dsRNA (target gene)-treated group and the control group
  2. *P < 0.0001 (dsEcR vs. dsLuciferase)
  3. **P < 0.0001 (dsUSP vs. dsLuciferase)