Fig. 4

Leishmania (Viannia) guyanensis isolate derived from a patient cured after treatment (IOC-L2335C) exhibited the lowest growth compared to the treatment failure and in vitro-selected resistant isolates. Growth curves after cell synchronization for the cure strain IOC-L2335C are indicated in black. The curve of the failure-derived sample (IOC-L2371F) is displayed in gray. The resistant isolate selected in vitro (IOC-L2335R) is presented in dark blue in the absence of Sb(III) and in dark red when the media contained 8 µM Sb(III). Three biological replicates were assayed. Dots indicate means, and bars indicate the standard errors. Two-way ANOVA was used followed by Tukey’s HSD post hoc test for IOC-L2335C. *P < 0.05, **P < 0.01, ***P < 0.001