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Fig. 3 | Parasites & Vectors

Fig. 3

From: The transmission ecology of Tahyna orthobunyavirus in Austria as revealed by longitudinal mosquito sampling and blood meal analysis in floodplain habitats

Fig. 3

Maximum likelihood phylogenetic trees of the coding regions for Tahyna orthobunyavirus nucleocapsid protein (a), polyprotein (b), and partial RNA-dependent RNA polymerase (c) isolated from pools of mosquitoes. Two isolates from Austria (in bold text marked with black circles) are compared to historical isolates from Europe and China, and the reference sequence of La Crosse orthobunyavirus was used as an outgroup. Terminal branch names list the GenBank accession number, the species of mosquito, the country, the isolate number, and the year when known. The accession numbers for the two isolates from Austria are Austria/OadD806/2019 (S: MZ245724, M: MZ245726, L: MZ245728) and Austria/OadD823/2019 (S: MZ245725, M: MZ245727, L: MZ245729). The trees are inferred over 500 bootstraps using the GTR + I (a), GTR + G + I (b), and GTR + G (c) substitution models. The lengths of the branches in substitutions per site are indicated by the scale bar. Because 858 nucleotides were not sequenced from isolate Austria/OadD806/2019 (MZ245728, nucleotide positions 2357–3215), this region was removed from the other sequences for analysis, maintaining codons in-frame

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