Fig. 2
From: Activation of the autophagy pathway decreases dengue virus infection in Aedes aegypti cells
Manipulation of the autophagy pathway with small molecules and after infection with DENV in Aag-2 cells. a Western blot results showing LC3-II levels after treatment with rapamycin and/or DMSO for 1, 3 and 6 h. b Western blot results showing LC3-II levels 2 days after rapamycin, DMSO and/or DENV infection. c Western blot results showing LC3-II level 2 days after treatment with 3-MA or Schneider’s Drosophila Media and/or infected with DENV. The relative ratios of the density, calculated using ImageJ software, compared between the bands of LC3-II and β-actin are shown on the top of the blot d DENV log PFUe/ml titer changes in both Aag-2 cell lysate and cell medium pretreated with DMSO, rapamycin or 3-MA for 3 h before DENV infection for 2 days. Holm-Å Ãdák's multiple comparisons test was used to calculate the P-value. Levels with the same uppercase letter are not significantly different. Abbreviations: DMSO, Dimethyl sulfoxide; 3-MA, 3-methyladenine; PFUe, plaque-forming unit equivalents