Fig. 3

Relative expression levels of Ae-CLIPB15 and Ae-CLIPB22 in adult mosquitoes following infection with Escherichia coli (a), Staphylococcus aureus (b) and Beauveria bassiana (c), and after physical injury (d). The control groups were injected with diethyl pyrocarbonate (Depc)-treated water. The abundances of messenger RNA (mRNA) of Ae-CLIPB15 and Ae-CLIPB22 were determined by quantitative real-time polymerase chain reaction (qPCR). The expression of these genes in the infection group was compared to that in the control group at 24 h post-infection. In the physical damage experiment, the control group was not injured. The mRNA abundance for Ae-CLIPB15 and Ae-CLIPB22 was determined by qPCR, and the mRNA expression levels of Ae-CLIPB15 and Ae-CLIPB22 in the injured groups were compared with those in the control group at 6 h and 12 h post-injury. The expression profiles were compared using two-way ANOVA in GraphPad software; data are presented as means ± SD, n = 3. ** P < 0.01, *** P < 0.001, **** P < 0.001. For other abbreviations, see Fig.  1