Fig. 3

Trichomonas vaginalis-induced ER stress response and ER stress-dependent apoptosis in SiHa cells. a SiHa cells were infected with T. vaginalis under the indicated conditions, and ER stress-related proteins were evaluated by western blotting analysis. b, c SiHa cells were pretreated with various concentrations of an ER stress inhibitor (4-PBA) for 2 h and subsequently infected with T. vaginalis at MOI 5 for 6 h. The ER stress-related (b) and apoptosis-related (c) protein levels were assessed using western blotting analysis. Anti-β-actin was used as an internal control. The data shown are representative of three independent experiments with similar results