Table 1 Target gene employed, size in base pairs of amplicons and cPCR and qPCR primer sequences used
Target gene | Size in base pairs (bp) | Primer names and sequences | References |
|---|---|---|---|
Conventional PCR | |||
Bartonella spp. 16S-23S ITS | 500–680 bp | BsppITS325s: 5′ CTTCAGATGATGATCCCAAGCCTTTGGCG 3′ BsppITS1100as: 5′ GAACCGACGACCCCCTGCTTGCAAAGCA 3′ | [19] |
Mycoplasma spp. (hemotropic group) 16S rRNA | 588 bp | Myco16S-322 s 5′ GCCCATATTCCTACGGGAAGCAGCAGT 3′ Myco16S-938as 5′ CTCCACCACTTGTTCAGGTCCCCGTC 3′ | [19] |
Anaplasma or Ehrlichia 16S rRNA | 420 bp | GEPs 5′ CTGGCGGCAAGCYTAACACATGCAAGTCGAACGGA 3′ GEPr 5′ CTTCTTCTRTRGGTACCGTCATTATCTTCCCYAYTG 3′ | [33] |
Babesia, Cytauxzoon, Hepatozoon, and Theileria spp. 18S rRNA | 663 bp | Piro18S-144 s 5′ ACCGTGCTAATTGTAGGGCTAATACA 3′ Piroplasma18S-722as 5′ GAATGCCCCCAACCGTTCCTATTAAC 3′ | [19] |
Real-time PCR | |||
Bartonella spp. 16S-23S ITS | 200–231 bp | BsppITS325s: 5′ CTTCAGATGATGATCCCAAGCCTTYTGGCG 3′ BsppITS543as: 5′ TAAAYTGGTGGGCCTGGGAGGACTTG 3′ Probe BsppITS500: 5′ FAM-GTTAGAGCGCGCGC TTGATAAG—IABkFQ 3′ | [34] |