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Fig. 1 | Parasites & Vectors

Fig. 1

From: In vitro characterization of Haemonchus contortus trehalose-6-phosphate phosphatase and its immunomodulatory effects on peripheral blood mononuclear cells (PBMCs)

Fig. 1

Cloning, expression and purification of HcGOB. a Amplification of HcGOB. Lanes: M, DNA marker DL 2000; 1, amplification products of the GOB gene; 2, negative control. b Lanes: M, DNA marker DL 10000; 1, digestion of pET-28a/HcGOB by enzymes. c–e Expression and purification of HcGOB. Lane M, standard protein molecular weight marker. c Lanes: 1, 2, pET-28a induced by IPTG for 0 and 5 h, respectively; 3–8, pET-28a/HcGOB induced by IPTG for 0, 1, 2, 3, 4 and 5 h, respectively. d Lanes: 1, supernatant of expression products; 2, inclusion body of expression products. e Lane 1: purification of recombinant (r) HcGOB

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Parasites & Vectors

ISSN: 1756-3305

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