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Fig. 9 | Parasites & Vectors

Fig. 9

From: In vitro characterization of Haemonchus contortus trehalose-6-phosphate phosphatase and its immunomodulatory effects on peripheral blood mononuclear cells (PBMCs)

Fig. 9

The rHcGOB protein activated the IL-10/TGF-β/STAT3 pathway in PBMCs. Cells were incubated with different concentrations of rHcGOB and with PBS (control) at 37 °C, and 5% CO2 for 72 h. a Transcription analysis of IL-10, TGF-β1, STAT3 in goat PBMCs. b The expression of IL-10, TGF-β1, p705-STAT3 was detected by western blot. The results presented here are representative of three independent experiments. Asterisks indicate significant differences at *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001 versus the control group. c Detection of nuclear translocation of phosphorylated STAT3 by IFA. The red color indicates localization of the target protein stained with Cy3 on PBMCs, and the blue color indicates the localization of nuclei stained with DAPI. Merge indicates the merged image of DAPI and Cy3. Scale bars: 20 μm

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