Fig. 2

a Silver-stained 5–20% SDS-PAGE gel showing replicated samples of saliva (4 µg/lane) from female (F1, F2, F3) and male (M1, M2, M3) Ornithodoros erraticus ticks. b Coomassie Blue-stained 5–20% SDS-PAGE gel showing pooled female saliva (F1 + F2 + F3). The gel lane was sliced into the three pieces, and the resulting gel slices (S1, S2, S3) were digested with trypsin and analysed by LC–MS/MS