Skip to main content
Fig. 1 | Parasites & Vectors

Fig. 1

From: DNA-based quantification and counting of transmission stages provides different but complementary parasite load estimates: an example from rodent coccidia (Eimeria)

Fig. 1

Development of an absolute qPCR quantification for Eimeria genome copy numbers. a Standard curves of Eimeria genomic DNA were generated from Ct values for E. falciformis and E. ferrisi DNA dilution series (n = 3) in the three cycling systems/instruments: ABI 7300 Real-Time PCR System (Applied Biosystems [ABI]; red circles), the MasterCycler® RealPlex2 cycler (Eppendorf; blue circles) and the CFX96™ Touch System (BioRad; green circles). Each point represents the linear decrease of the Ct values with increase of template along the curve. The linear equation, i.e. coefficient of determination (R2) is reported for each curve. b Eimeria genome copies per nanogram of gDNA are proportional to the counted oocysts with application of standard curve. Abbreviations: Ct, threshold cycle

Back to article page