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Fig. 2 | Parasites & Vectors

Fig. 2

From: DNA-based quantification and counting of transmission stages provides different but complementary parasite load estimates: an example from rodent coccidia (Eimeria)

Fig. 2

Eimeria quantification during the infection. Mice were infected with E. ferrisi (isolate Brandenburg64) and the infection course was tracked by: a Eimeria genome copies per gram of faeces estimated by qPCR, and b oocysts per gram of faeces estimated by flotation and counting. Note the different scales of the y-axes in these plots that indicate different orders of magnitude for both measurements, i.e. more genome copies than expected from the number of oocysts. In a, the shape represents the detection of Eimeria DNA in the faecal samples, as confirmed by melting curve analysis. c Weight loss during the infection relative to dpi 0. Boxplots represent the OPG or Eimeria genome copies per gram of faeces quantification for 22 mice during the infection. The centre line represents the median; box limits are the first and third quartiles; and the whiskers mark the interquartile range. Each filled circle represents an individual faecal sample (n = 242), colour-code by number of dpi. Grey lines link measurements for the same mouse individual. Statistical significance was evaluated against the same number of zero values with a Mann–Whitney U-test, and P-values were adjusted using Bonferroni correction. Asterisks indicate significance: **adjusted-P < 0.001, ***adjusted-P < 0.001. Abbreviations: ns, Not significant

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