From: Overview of paratransgenesis as a strategy to control pathogen transmission by insect vectors
Transformed microbes used + effector genes | Insect vectors | Experimental results with transformed microbes | Key references |
---|---|---|---|
R. rhodnii + pRr1.1 shuttle plasmid with antibiotic resistance | R. prolixus | First proof of concept in insect vectors. Successful transformation and maintenance of symbiotic bacteria in vector | |
R. rhodnii + RrThioCec-(transformed R. rhodnii + cecropin A) | R. prolixus | Elimination or reduction of T. cruzi in vivo | |
R. rhodnii + rDB3 antibody fragment | R. prolixus | Secretion of antibody fragments into gut lumen in vivo | [72] |
Corynebacterium sp. + rDB3 | Triatoma infestans | Secretion of antibody fragments into gut lumen in vivo | |
R. rhodnii + recombinant Arthrobacter luteus β-1,3-glucanase | Potential additional effector in R. prolixus in vivo | Lysates of β-1,3-glucanase transformed R. rhodnii kill T. cruzi in vitro | [256] |
R. rhodnii + rDB3 antibody fragment | R. prolixus | Semi-field simulation of transgenic bacteria spread in Cruzigard | [73] |
E. coli + dsRHBP + dsCAT | R. prolixus | Proof of concept with transgenic symbiont -mediating RNAi in adults and nymphs | [111] |
R. rhodnii + dsNP1 R. rhodnii + dsNP2 R. rhodnii + dsVg | R. prolixus | Proof of concept with transgenic symbiont -mediating RNAi in aposymbiotic nymphs | |
Sodalis glossinidius-fp | Glossina morsitans morsitans | Transfection study to progeny | |
S. glossinidius-gfp | G. m. morsitans Glossina fuscipes fuscipes | Reciprocal transinfection occurs with no fitness costs | [270] |
S. glossinidius + Nb_An46 | G. m. morsitans | The nanobody was expressed in vivo by the transformed Sodalis | |
S. glossinidius-gfp | G. m. morsitans | Much impoved bacterial colonisation of progeny | [291] |
S. glossinidius | G. m. morsitans | Paratransgenesis combination advocated with sterile insect technique | [294] |
Bacillus subtilis-gfp | Phlebotomus argentipes | Laboratory transfection study in larvae and transstadial transmission | |
Enterobacter cloacae-DR | Phlebotomus papatasi | Laboratory transfection study with limited tranasstadial transmission | [325] |