Skip to main content

Table 1 Primers used for the amplification of seven DNA regions

From: Infection of the Asian gray shrew Crocidura attenuata (Insectivora: Soricidae) with Sarcocystis attenuati n. sp. (Apicomplexa: Sarcocystidae) in China

DNA region

Primer name

Primer sequence (5′–3′)

Reference

18S rDNA

ERIB1a

ACCTGGTTGATCCTGCCAG

[6]

 

S2b

CTGATCGTCTTCGAGCCCCTA

[7]

 

S3a

TTGTTAAAGACGAACTACTGCG

[7]

 

Bb

GATCCTTCTGCAGGTTCACCTAC

[8]

ITS1

ITSFa

GTTCCGGTGAATTATTCGGACTGTT

This study

 

ITSRb

GATGATTCCCTGAATTCTGCAATTC

This study

cox1

526F1a

TCCTTCCTGGCGTACAACAATCAT

This study

 

1209R1b

GGGGCATGACATTGAAAGCAAGTA

This study

cox3

COX3F1a

GCTTTAAACGTATTGTATTTCAATA

This study

 

COX3R1b

TCAACCATAGACATTCTATGAAATG

This study

cytb

1080CYTBF2a

ATGAGTTTAGTGCGAGCACATTT

This study

 

1080CYTBR2b

TTAATATAGACATACAGCTAAGCTTGTGA

This study

rpoB

RpoBFa

ATTTTTGTGGATATGATTTTGAAGATGC

[9]

 

RpoBRb

TTTCCATATCTTCCACATAATTTATCTC

[9]

clpC

ClpCF-1a

GGAGCACCACCTGGGTATGT

This study

 

ClpCR-1b

CGAGCTCCATATAAAGGATGATAAG

This study

  1. The primers used in this study for internal transcribed spacer region 1 (ITS1), cytochrome oxidase subunit 1 (cox1), cox3, cytochrome b (cytb), and caseinolytic protease C (clpC) were designed using OLIGO 5.0 (National BioScience, Plymouth, MN) based on the highly conserved regions of the corresponding sequences for Sarcocystis spp., Toxoplasma gondii, Besnoitia besnoiti and Hammondia heydorni deposited in GenBank
  2. 18S rDNA 18S ribosomal DNA, rpoB RNA polymerase beta subunit
  3. aForward primer
  4. bReverse primer