Table 1 Sequence of primers used in qPCR (accession number/reference), size (base pairs [bp]), and melting temperature (Tm, in °C) of amplified products, and MgCl2 concentrations used in each reaction
From: Local immune response of Canarian Majorera goats infected with Teladorsagia circumcincta
Primers 5′ and 3′ | Size (bp) | MgCl2 (mM) | Tm (°C) | Accession number/reference | |
|---|---|---|---|---|---|
Β-act | CCAACCGTGAGAAGATGACCC CCCAGAGTCCATGACAATGCC | 122 | 5 | 85.0 | AF481159 |
IL-2 | GTGAAGTCATTGCTGCTGGA TGTTCAGGTTTTTGCTTGGA | 202 | 3 | 81.0 | Craig et al. (2007) [52] |
IL-4 | GCTGGTCTGCTTACTGGTATG CGATGTGAGGATGTTCAGC | 100 | 5 | 80.0 | FJ936316 |
IL-10 | GTGGAGCAGGTGAAGAGAGTC TGGGTCGGATTTCAGAGG | 198 | 3 | 82.0 | AF458378 |
INF-γ | AGATAACCAGGTCATTCAAAGGAG GGCGACAGGTCATTCATCAC | 180 | 3 | 82.5 | U34232 |
IL-17 | TGCTACTGCTTCTGAGTCTGGTGGC TGACCCTCACATGCTGTGGGAAGTT | 111 | 0 | 83.5 | Yan et al. (2011) |