Table 3 Details of the result of the specimens submitted to molecular biology analysis and homology with the reference sequences available on GenBank using BLAST

-Specimens using for confirming MALDI-TOF MS identification

 Ae. aegypti^a

3

100%

Ae. aegypti (100%)

MN299016

 Ae. albopictus^a

3

99.7–100%

Ae. albopictus (99.7–100%)

MN299017, MK995332

 Cx. quinquefasciatus^b

3

99–100%

Cx. Quinquefasciatus (99.6–100%)

MN299021, MK575480

 Cx. sitiens^a

2

100%

Cx. sitiens 99.8%

MF179212

 An. aconitus^a

2

99.8%

An. aconitus (100%)

HQ877378

 An. annularis^a

1

99.5%

An. annularis (96.6%)

KF406655

 An. dirus^a

2

100%

An. dirus (99.7%)

JX219732

 An. minimus s.l^a

2

100%

An. harrisoni (99.8–100%)

HQ877377

 An. minimus s.l.^a

2

100%

An. minimus (99.4%)

HQ877337

 An. jamessi^a

2

89.2–100%

An. jamessi (99.7–99.8%)

MT380518, MT871938

 An. pediteaniatus^a

3

96–99.5%

An. pediteaniatus (99.3–99.8%)

LC333267

 An. sinensis^a

2

100%

An. sinensis (99.7%)

MG816536

 An. vagus^a

2

100%

An. vagus 99.7%

MK685245

 An. maculatus^a

3

100%

An. maculatus 99.8%

KT382822

 An. varuna^a

2

100%

An. varuna (100%)

HQ877380

Specimens used for database update

 An. aconitus^a

2

90.4–99.8%

An. aconitus (99.8–100%)

HQ877378

 An. annularis^a

1

-

An. annularis (96.6%)

KF406655

 An. dirus^a

3

89.7–100%

An. dirus (99.5–99.7%)

JX219732

 An. minimus s.l.^a

4

90–99.84%

An. harrisoni (99.8–100%)

HQ877377

An. minimus (99.4%)

HQ877337

 An. barbirostris^a

1

99.7%

An. barbirostris (99.7%)

AB971312

 An. jamessi^a

4

89.2–100%

An. jamessi (99.7–99.8%)

MT380518, MT871938

 An. pediteaniatus^a

4

96–99.5%

An. pediteaniatus (99.3–99.8%)

LC333267

 An. sinensis^a

1

98.7–98.9%

An. sinensis (99.5–99.7%)

MG816536, MG816562, KX779641

 An. vagus^a

3

98.2–98.7%

An. vagus (99–99.7%)

MK685245, MH425442, MF179262

 An. maculatus^a

2

100%

An. maculatus 99.8%

KT382822

 An. varuna^a

1

-

An. varuna (100%)

HQ877380

 An. splendidus^c

2

99.8%

An. splendidus (98.4–98.7%)

MK685253

 Culex spp.^a

15

Cx. tritaeniorhynchus (99.3–100%)

MF179219, MF179221, MF179231, MF179232

Cx. fuscocephala (100%)

HQ398887

Cx. pseudovishnui (97.9–98.2%)

AB738092, LC054483

Cx. pallidothorax (98%)

MF179281

Cx. vishnui (99.2%)

MF179240

Specimens with discrepancies between morphological and MALDI-TO MS identification

 An. aconitus^a

1

-

An. varuna (100%)

HQ877380

 An. dirus^a

1

-

An. sinensis (99.5%)

MG816562

 An. minimus^a

4

99–100%

An. varuna (99–100%)

HQ877380

 An. jamesi^a

1

-

An. maculatus (93.8%)

KT382822

 An. pediteniatus^a

2

99.5%

An. sinensis (99.4–99.5%)

MG816562, KX779641

 An. maculatus^a

9

100%

An. maculatus (93.6%)

KT382822

 Culex sp.^a

1

-

An. minimus A (99.5%)

HQ877337

 Culex sp.^a

1

-

An. jamesii (99.7%)

MT871938

 Culex sp.^a

1

-

An. dirus (99.7%)

JX219732