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Fig. 1 | Parasites & Vectors

Fig. 1

From: Vaccination of cattle with synthetic peptides corresponding to predicted extracellular domains of Rhipicephalus (Boophilus) microplus aquaporin 2 reduced the number of ticks feeding to repletion

Fig. 1

Timeline of experiment and western blot showing immune response to native tick protein after vaccination with peptides. Timeline represents days, counted from the first vaccination. Green dots represent vaccinations, with the first three vaccinations (days 0, 21, and 42) done at 3-week intervals using peptides conjugated to KLH, and the fourth vaccination (day 63) consisting of peptides alone. Blue triangles indicate blood samples taken before the initial vaccination (pre-bleed), 2 weeks after each vaccination, and weekly during the tick feed (days 0, 14, 35, 56, 70, 77, 84, 91, 98). Red diamonds indicate dates ticks were placed on (day 70) and collected as detached repletes (days 91–96). Orange stars on the timeline correspond to lanes on the western blot showing reactivity of serum from representative peptide-vaccinated calf C1554, with native tick protein showing reactivity to a 50–55 KDa band in this calf; the other two vaccinated calves showed similar reactivity

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