Table 2 Molecular identification of species using both ITS2 and COII comparisons
Sequence group | No. of samples (ITS2; COII) | Sequence length in bp (ITS2; COII) | Molecular species ID | Morphology species ID (no.) | No. of sequences identified molecularly | No. of correctly identified specimens based on morphology | % of correctly identified specimens based on morphology |
|---|---|---|---|---|---|---|---|
Group 1 | 22; 21 | 717; 687 | An. dirus | d (22) | 22 | 22 | 100.00 |
Group 2 | 18; 18 | 457; 674 | An. peditaeniatus | s (18) | 18 | 0 | 0.00 |
Group 3 | 17; 7 | 329; 685 | An. maculatus | m (16), phi (1) | 17 | 16 | 94.12 |
Group 4 | 2; 2 | 353; 685 | An. philippinensis | phi (2) | 2 | 2 | 100.00 |
Group 5 | 244; 213 | 400; 718 | An. kochi | k (242), v (1), s (3) | 246 | 242 | 98.37 |
Group 6 | 11; 12 | 568; 686 | An. vagus | v (7), k (5) | 12 | 7 | 58.33 |
Group 7 | 9; 9 | 467/591; 690 | An. tessellatus | t (5), k (4) | 9 | 5 | 55.56 |
Group 8 | 4; 3 | 480; 686 | An. nitidus | s (4) | 4 | 0 | 0.00 |
Group 9 | 59; 53 | 356; 690 | An. nivipes | phi (59) | 59 | 0 | 0.00 |
Group 10 | 8; 8 | 472; 687 | An. argyropus | s (8) | 8 | 0 | 0.00 |
Group 11 | 4; 4 | 469; 686 | An. sinensis | s (4) | 4 | 4 | 100.00 |
Group 12 | 14; 14 | 508; 687 | An. nigerrimus | s (14) | 14 | 0 | 0.00 |
Group 13 | 0; 27 | 0; 691 | An. barbirostris | s (26), k (1) | 27 | 0 | 0.00 |
Total | 412; 391 | – | – | – | 442 | 298 | 67.42 |