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Table 2 Molecular identification of species using both ITS2 and COII comparisons

From: Morphological and molecular identification reveals a high diversity of Anopheles species in the forest region of the Cambodia–Laos border

Sequence group No. of samples (ITS2; COII) Sequence length in bp (ITS2; COII) Molecular species ID Morphology species ID (no.) No. of sequences identified molecularly No. of correctly identified specimens based on morphology % of correctly identified specimens based on morphology
Group 1 22; 21 717; 687 An. dirus d (22) 22 22 100.00
Group 2 18; 18 457; 674 An. peditaeniatus s (18) 18 0 0.00
Group 3 17; 7 329; 685 An. maculatus m (16), phi (1) 17 16 94.12
Group 4 2; 2 353; 685 An. philippinensis phi (2) 2 2 100.00
Group 5 244; 213 400; 718 An. kochi k (242), v (1), s (3) 246 242 98.37
Group 6 11; 12 568; 686 An. vagus v (7), k (5) 12 7 58.33
Group 7 9; 9 467/591; 690 An. tessellatus t (5), k (4) 9 5 55.56
Group 8 4; 3 480; 686 An. nitidus s (4) 4 0 0.00
Group 9 59; 53 356; 690 An. nivipes phi (59) 59 0 0.00
Group 10 8; 8 472; 687 An. argyropus s (8) 8 0 0.00
Group 11 4; 4 469; 686 An. sinensis s (4) 4 4 100.00
Group 12 14; 14 508; 687 An. nigerrimus s (14) 14 0 0.00
Group 13 0; 27 0; 691 An. barbirostris s (26), k (1) 27 0 0.00
Total 412; 391 442 298 67.42
  1. Morphologically based species identification included: d, An. dirus; s, An. sinensis; m, An. maculatus; phi, An. philippinensis; k, An. kochi; v, An. vagus; t, An. tessellatus. Number and percentage of correctly identified morphological specimens are calculated from the number of sequences that were molecularly identified per species