Fig. 6
Upregulated Acan-Gal-1 plays a defensive role against oxidative stress. a Oxidative stress assays using H2O2 in N2 and lec-1 mutant worms expressing pCe-lec-1::Acan-gal-1::rfp. b Relative expression levels of apoptosis genes in lec-1 mutant worms expressing pCe-lec-1::rfp and in lec-1 mutant worms expressing pCe-lec-1::Acan-gal-1::rfp. c Oxidative stress assays using H2O2 in ced-3 mutant worms expressing pCe-lec-1::Acan-gal-1::rfp or pCe-lec-1::rfp. d Oxidative stress assays using H2O2 in ced-3 mutant worms with lec-1 RNAi. e Oxidative stress assays using H2O2 in fat-6;fat-7 double-mutant worms expressing pCe-lec-1::Acan-gal-1::rfp or with lec-1 RNAi. The worms were counted as described in “Methods” section. The error bars indicate standard deviation. *P < 0.05; **P < 0.01; ***P < 0.001