Fig. 10

Characterisation of phlebovirus-like sequences from I. holocyclus ticks. a Schematic of the organisation of PVL1. The position and coding direction of two ORFs which were also found in PVL3 are indicated by arrows and nucleotide numbers. N, nucleocapsid-like protein gene. b MAFFT alignment of the 237 amino acid proteins encoded by PVL1 and PVL3 with nucleocapsid proteins of selected phleboviruses. Rectangles above sequence indicate elements of secondary structure identified for Rift Valley Fever virus (RVFV) nucleocapsid protein (alpha helices, a1–a13). Boxes enclose secondary structures predicted in PVL1 and PVL3 sequences using Phyre2. Yellow, n-terminus arm domain; green, N-lobe of globular core domain; magenta, C-lobe of globular core domain; grey, c-terminus not involved in RNA binding. Brown box at N-terminus of PVL1 indicates predicted beta strand at the expected position of alpha helix 3. UUKV, Uukuniemi virus; SFNV, Sandfly fever Naples virus; MUKV, Mukawa virus; OKTV, Okutama tick virus. c Alignment of a second conserved protein identified in both PVL1 and PVL3. d Maximum likelihood analysis of amino acid sequences of phlebovirus nucleocapsid proteins and proteins of PVL1 and PVL3. Maximum-likelihood analysis was performed using the LG + G + I model in Mega7. The percentage of trees in which the associated taxa clustered together is shown next to the branches. Grey vertical line indicates sequences identified in I. holocyclus ticks from Australia, black line indicates sequences from I. uriae ticks in Antarctica