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Fig. 5 | Parasites & Vectors

Fig. 5

From: Extended characterisation of five archival tick-borne viruses provides insights for virus discovery in Australian ticks

Fig. 5

In vitro replication of NUGV in vertebrate and tick cell lines. a Growth kinetics analysis of NUGV in ISE6 and BSR cells. Viral titres in supernatants from three replicate wells were determined by TCID50 method after titration on BSR cells. b Detection of dsRNA in NUGV-infected BSRs at various time points post-infection. c Detection of dsRNA in NUGV-infected ISE6 cells at various time points post-infection. Blue, Hoechst nuclear stain; green, anti-dsRNA immunolabelling by MAVRIC. Images taken at 40 × magnification

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