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Fig. 7 | Parasites & Vectors

Fig. 7

From: Extended characterisation of five archival tick-borne viruses provides insights for virus discovery in Australian ticks

Fig. 7

Sequence and in vitro analysis of the tick-borne phlebovirus CMCV. a Annotation of CMCV L protein and glycoprotein precursor. Numbers indicate relevant amino acid positions. Green: Gn protein. Magenta: Gc protein. The predicted molecular weight of each mature glycoprotein is indicated in brackets. Predicted signal peptidase cleavage sites preceding Gn (GEL-YF) and Gc (VEL-CS) are indicated by solid lines. An arrow indicates the position of a predicted furin cleavage site (RSKR) in the Gn protein. N, N-terminus; DUF, domain of unknown function (pfam: DUF3770); RdRp, RNA-dependent RNA polymerase; TM, transmembrane domain; S, signal peptide; Y, predicted N-glycan. b Summary of CMCV sequence data for the L and M segment generated in this study and similarity to Precarious point virus. CMCV S segment sequence was published in [15]. c SDS-PAGE analysis of purified CMCV. RNP, ribonucleoprotein complex; Gn/Gc, mature glycoproteins. d Growth kinetics of CMCV in vertebrate (BSR, baby hamster kidney) and tick (ISE6, Ixodes scapularis) cells. Viral titres in supernatants from three replicate wells were determined by TCID50 method after titration on Vero cells

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