Extended characterisation of five archival tick-borne viruses provides insights for virus discovery in Australian ticks

Table 2 Primer sets used in this study

Primer name	Sequence (5′–3′)	Product size (bp)	Annealing temperature (°C)
PVL1 Forward	TCACACCTTGGGCATTCTCC	679	51
PVL1 Reverse	AAACCGAGACACCATCACCC	679	51
PVL2 Forward	CTACTGACATGCGAGTTCC	185	45
PVL2 Reverse	CAGTCGACGAGACACAACCG	185	45
PVL3 Forward	GATTTTGGTCATGGCAGC	435	45
PVL3 Reverse	CACTTGGCATACAGCTGG	435	45
Rhabdo-like F	CCTTAGGCCGACGTCTTATCC	263	51
Rhabdo-like R	GAGCAGTTCTTTGGCTTGCC	263	51

ISSN: 1756-3305