Extended characterisation of five archival tick-borne viruses provides insights for virus discovery in Australian ticks

Table 3 Reactivity of pan-flavivirus mAbs and MAVRIC against SREV and GGYV in fixed-cell ELISA

Antibody	Reactive protein	Reactivity in fixed-cell ELISA^a			References
Antibody	Reactive protein	WNV_KUN	GGYV	SREV	References
4G2	Envelope	+ + +	+ + +	+ +	[71]
M2-1E7	Envelope	+ + +	+ +	−	[72]
6B6C	Envelope	+ + +	+ +	+	[73]
3H6	Envelope	+ + +	+ +	+	[74]
1D1	Envelope	+ + +	+ +	+	[75]^b
P3H8	Envelope	+ + +	+	+	[76]
MAVRIC	dsRNA	+ +	−^c	−^c	[23]

^a+ + + , OD_405nm ≥ 2 for all tested dilutions; + + , OD_405nm ≥ 1 for all tested dilutions; +, OD_405nm < 1 for all tested dilutions; −, OD_405nm ≤ mock OD for all tested dilutions
^bmAb prepared by A.K. Falconar, London School of Hygiene and Tropical Medicine, London, UK
^cGreater than mock OD but beneath the threshold for a positive score (2 × mock OD_405nm). Negative for MAVRIC in fixed cell ELISA using both 20% acetone and 4% formaldehyde with 0.5% Triton X-100 fixation

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