Extended characterisation of five archival tick-borne viruses provides insights for virus discovery in Australian ticks

Table 4 Summary of sequence data generated for Nugget virus

Expected segment^a	Gene^b	Accession number	Segment length (nt)	Protein length (aa)	% Pairwise identity with GIV (nt/aa)^c	% Pairwise identity with SBaV (nt/aa)^d
1	VP1 (Pol)	OK396633	3776	1258	69.4/80.3	–
2	VP3 (T2)	OK396634	2559	852	74.9/90.4	–
3	VP4 (CaP)	OK396635	1645	547	65.3/71.9	96.4/97.0
4	NS1 (TuP)	OK396636	1548	515	63.9/69.9	–
5	VP2 (OC1)	OK396637	1458	486	53.4/59.7	–
6	VP5 (OC2)	OK396638	1229	409	66.0/70.4	98.4/99.0
7	VP7 (T13)	OK396639	988	329	75.6/91.2	–
8	NS2 (ViP)	OK396640	1143	372	68.3/76.1	98.0/99.2
9	VP6 (Hel)	OK396641	959	314	64.9/51.4	–
10	NS3	OK396642	566	157	66.9/72.0	98.0/100.0

^aSegment predictions are based on GIV segment designations [77]
^bViral protein designations (VP) are based on Bluetongue virus nomenclature as per [78]. Pol, RNA-dependent RNA polymerase; T2, conserved major subcore protein with T = 2 symmetry; CaP, capping enzyme; TuP, tubule forming protein; OC1, outer capsid protein 1; OC2, outer capsid protein 2; T13, major core protein with T = 13 symmetry; ViP, viral inclusion body protein; Hel, ssRNA- and dsRNA-binding helicase; NS, non-structural protein
^cGIV nucleotide sequences (NC_014522 to NC_014531), amino acid sequences (YP_003896058 to YP_003896068)
^dSBaV nucleotide sequences (EU685329 to EU685333), amino acid sequences (ACD38335 to ACD38337 and ACD38339)

ISSN: 1756-3305