Seasonality and transmissibility of Plasmodium ovale in Bagamoyo District, Tanzania

Parasites & Vectors

Table 2 Results of human-to-mosquito transmission experiments from subjects recruited after screening who were positive for P. ovale infection by PCR on day of DFA (direct feeding assay)

Subject ID	Blood PCR^a on day of DFA		No. of positive midguts	Midgut PCR at D8 post-feeding		No. of PCR+ mosquito pools at D14 post-feeding		P. ovale-positive mosquitoes only^b	P. falciparum- & P. ovale-positive mosquitoes
Subject ID	P. falciparum	P. ovale	No. of positive midguts	P. falciparum	P. ovale	P. falciparum	P. ovale	P. ovale-positive mosquitoes only^b	P. falciparum- & P. ovale-positive mosquitoes
MqTZ-2312	++	++	1/3	ND	ND	0/7	6/7	✓	✗
MqTZ-2390	+++	+	3/10	++	+	5/6	4/6	✗	✓
MqTZ-2394	+++	+++	3/47	–	+	ND	ND	✓	✗
MqTZ-2396	+++	++	1/10	++	+/–	0/6	5/6	✓	✗
MqTZ-2431	–	+++	3/48	–	++	ND	ND	✓	✗
MqTZ-2499	+++	+++	5/10	++	+	1/14	8/14	✗	✓
MqTZ-2556	–	+	1/34	+	++	ND	ND	✗	✗
MqTZ-2563	–	+++	0/43	ND	ND	ND	ND	✗	✗

In all DFA experiments, mosquito midguts were dissected at day 8 (D8) post-feeding for detection of parasite oocysts by microscopy. In four subjects (subject ID in boldface), additional mosquitoes were held until day 14 (D14) post-feeding to assess sporozoite development by qPCR
^aFor qPCR results, +++ denotes a mean C_t value ≤ 32, ++ denotes a mean C_t value > 32 but < 40, + denotes a mean C_t value ≥ 40, and – indicates that no qPCR product was detected. For qPCRs with a C_t ≥ 40, only those with a confirmatory follow-up qPCR are reported
^bPlasmodium ovale infection status determined at D14

ISSN: 1756-3305