Tick-borne pathogens in neotropical animals in Trinidad, West Indies

Parasites & Vectors

Table 2 Primers and their sequences used in this study

Primers	Sequence 5′-3′	Target gene	Target genera	Amplicon size (bp)	References
RLB-F2 RLB-R2	ACACAGGGAGGTAGTGACAAG CTAAGAATTTCACCTCTGACAGT	18S rRNA	Babesia/Theileria	540	[32]
Piroplasmid-F Piroplasmid-R	CCAGCAGCCGCGGTAATTC CTTTCGCAGTAGTTYGTCTTTAACAAATCT	18S rRNA	Babesia/Hepatozoon	350	[33]
PIROA PIROB	AATACCCAATCCTGACACAGGG TTAAATACGAATGCCCCCAAC	18S rRNA	Babesia	400	[34]
16S8FE B-GA1B	AGAGTTGGATCMTGGYTCAG CGAGTTTGCCGGGACTTYTTC	16S rRNA	Ehrlichia/Anaplasma	500	[37]
E.c 16S-fwd E.c. 16S-rev	TCGCTATTAGATGAGCCTACGT GAGTCTGGACCGTATCTCAG	16S rRNA	Ehrlichia/Anaplasma	123	[38]
EHR16SD EHR16SR	GTACCYACAGAAGAAGTCC TAGCACTCATCGTTTACAGC	16S rRNA	Ehrlichia/Anaplasma	345	[40]
443F 781R	GCTATGTCTGCATTCTATCA CCACCATGAGCTGGTCCCC	gltA	Bartonella	340	[35]
ssrAF ssrAR	GCTATGGTAATAAATGGACAATGAAATAA GCTTCTGTTGCCAGGTG	ssrA	Bartonella	300	[35]
rico173F rico173R	CGACCCGGGTTTTATGTCTA ACTGCTCGCCACTTGGTAGT	gltA	Rickettsia	133	[36]

ISSN: 1756-3305