Fig. 4
From: Increased biting rate and decreased Wolbachia density in irradiated Aedes mosquitoes
Decreased Wolbachia titer in irradiated SANG and ARwP Ae. albopictus. a A 45-Gy dose was applied at three different pupal ages (26, 36, and 46 ± 2 h); quantitative PCR analysis was then performed using primers targeting the specific Wolbachia strains characterizing the two Ae. albopictus populations and analyzing 6 ± 1-day-old females. b A 45-Gy dose was applied to pupae aged 36 ± 4 h; qPCR analysis was then carried out studying two female ages (6 and 13 ± 1 days). Data were normalized using the actin gene as host reference. Within each infection type and female age, different letters indicate statistically significant differences between treatments (one-way ANOVA followed by Tukey's test: P < 0.05)