Fig. 5
From: Increased biting rate and decreased Wolbachia density in irradiated Aedes mosquitoes
Decreased Wolbachia titer in the ovaries of irradiated SANG and ARwP Ae. albopictus. A 45-Gy dose was applied to 36 ± 4-h-old pupae. a qPCR analysis was performed on the dissected ovaries of 6 and 13 ± 1-day-old females using primers targeting the specific Wolbachia strains characterizing the two Ae. albopictus populations. Data were normalized using DNA (2 μl of purified DNA per reaction). b qPCR analysis was performed on bodies lacking ovaries of 6- and 13 ± 1-day-old females using primers targeting the specific Wolbachia strains characterizing the two Ae. albopictus populations. Data were normalized using the actin gene as host reference. Within each infection type and female age, different letters indicate statistically significant differences between treatments (one-way ANOVA followed by Tukey's test: P < 0.05)