Skip to main content
Fig. 7 | Parasites & Vectors

Fig. 7

From: A review on the diagnosis of animal trypanosomoses

Fig. 7

Molecular and serological tests for the detection of trypanosomes and trypanosomoses: a Ethidium bromide-stained electrophoresis gel of a monospecific PCR; the result can be considered to be positive when a visible PCR product exhibits the specific weight expected (here: lanes 2, 3, 6–10, 12, 14, 16); otherwise, when the product is non-specific (lane 17) or non-visible (lanes 1, 4, 5, 11, 13, 15), the PCR is negative. Lane 18 is the DNA ladder. b Ethidium bromide-stained electrophoresis gel of a multi-specific PCR based on the amplification of the internal transcribed spacer 1 (ITS1); species-specific results are deduced from the weight of the visible PCR products obtained (here: lanes 1, 12 are the DNA ladder; lanes 2–4 are T. congolense; lanes 5–7 are Trypanozoon; lane 8 is T. theileri; lane 9 is T. simiae; lanes 10, 11 are T. vivax. c Trypanosoma vivax antibody detection ELISA plate; first 2 rows are blanks (A, B), positive controls (C, E, G) and negative controls (D, F, H); all samples are tested in duplicate and appear to be positive, doubtful or negative, according to their mean optical density. d Picture of the card of a CATT/T. evansi exhibiting parasite agglutinations in the positive control and samples 163 and 017; other samples are considered to be negative

Back to article page