A review on the diagnosis of animal trypanosomoses

Parasites & Vectors

Table 2 Summary of diagnostic uses for main Tryanosoma species responsible for animal African trypanosomosis and non-tsetse transmitted animal trypanosomosis

Target	Technique^a	Subgenus Nannomonas	Subgenus Duttonella	Subgenus Trypanozoon
Target	Technique^a	Trypanosoma congolense	Trypanosoma vivax	Trypanosoma brucei brucei	Trypanosoma evansi	Trypanosoma equiperdum
Parasite	Wet blood films	+^b	+	+	+ Trypanozoon
	HTC/BCT	+ + +	+ + +	+ + +	+ + + Trypanozoon
	mAECT	−	−	−	−	−
	Inoculation of rodents	+	0	+	+ +	+
DNA	Pan-species primers (ITS1)	+ +	+ +	+ +	+ + Trypanozoon	+
	Genus/species/type-specific primers	+ + +	+ + +	+ + +	+ + + Trypanozoon	+ +
	Specific primers among Trypanozoon			0	+	0
Antibodies	ELISA on WCLSA	+ + +	+ + +	+ + +	+ + + Trypanozoon	+
	Protein-specific ELISA	Under test	Under test	0	+	0
	Agglutination test (CATT)	0	0	+	+ + +	0
	IFAT	−	−	−	−	+
	CFT	0	0	0	0	+ +
	TL	0	0	0	+	0

^aSee Abbreviation List for the full description of each abbreviation
^bQualitative uses: + + + = well adapted and used; + + = adapted and used; + = can be used; 0 = not used in practice

ISSN: 1756-3305