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Fig. 3 | Parasites & Vectors

Fig. 3

From: Inhibition of COX-2 ameliorates murine liver schistosomiasis japonica through splenic cellular immunoregulation

Fig. 3

NS398 affected the number of macrophages, but not neutrophils and MDSC in the spleen of Sj-infected mice. Spleens from non-infected and infected mice with or without NS398 treatment were harvested and cells were isolated. a Spleen cells were stained with dead-APC-A750, CD45-PB450 or KO525, CD11b-PE, F4/80-FITC, Ly6G- PC7, and Gr-1-APC antibodies. F4/80 + CD11b+ cells (macrophage), Ly6G+CD11b+ cells (neutrophil or PMN) or Gr-1+ CD11b+ cells (MDSCs) were analyzed, and data shown are gated on CD45+ live cells. Ly6G+ cells in MDSCs (PMN-MDSCs) were also analyzed. The expression of macrophages (b) and neutrophils (c) in CD45+ cells in the spleen was evaluated. MDSCs in CD45+ cells (d) and PMN-MDSCs in MDSCs or in CD45+ cells (e) in the spleen were evaluated. Data are expressed as the mean ± SD of indicated numbers of mice from different groups (Student’s t-test, statistic P-value is marked in the figures)

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