Fig. 5

NS398 decreased Sj infection-triggered Tfh and Tfr. a Isolated spleen cells were stained with CD3-FITC, CD4-KO525, Foxp3-PC5.5, CXCR5-APC, PD-1-PC7. Foxp3⁺ or Foxp3⁻ cells were analyzed and data shown are gated on CD3⁺CD4⁺ T cells. CXCR5⁺ PD-1⁺ cells (Tfh) were analyzed, and data shown are gated on Foxp3⁺ or Foxp3⁻ CD4⁺ T cells, respectively. Numbers represent the percentage of the boxed population. b The expression of Foxp3⁺ or Foxp3⁻ cells in CD3⁺CD4⁺ T cells in the spleen was evaluated. c CXCR5⁺ PD-1⁺ cells or d CXCR5⁺ PD-1⁺ T cells (Tfr) in spleen were evaluated. Data are expressed as the mean ± SD (Student’s t-test, statistic P-value is marked in the figures)