Table 1 Primers, target genes and PCR conditions used in this study
Pathogen | Primers | Annealing temperature (°C) | Target gene | Product size (bp) | References |
|---|---|---|---|---|---|
Acanthocheilonema reconditum | AR COI-F1: AGTGTTGAGGGACAGCCAGAATTG AR COI-R1: CCAAAACTGGAACAGACAAAACAAGC | 59 | cox1 | 200 | [25] |
Dirofilaria spp. | COXdirHRMF: AGTATGTTTGTTTGAACTTC COXdirHRMR: AACGATCCTTATCAGTCAA | 52 | cox1 | 256 | [26] |
Wolbachia pipientis | Wol1_fwd: CCTGTACTATATCCAAGAATTACTG Wol1_R: ACTATCCTTTATATGTTCCATAATTTC | 57.5 | ftsZ | 267 | [27] |