Table 1 Details of the PCR primers used in the present study, with the respective positive control DNA used in the reactions

18S rRNA

Hepatozoon spp.

HEP2 144–169 (F)

GGTAATTCTAGAGCTAATACATGAGC

574

[41]

Hepatozoon canis

HEP2 743–718 (R)

ACAATAAAGTAAAAAACAYTTCAAAG

HAM-1(F)

GCCAGTAGTCATATGCTTGTC

1750

[42]

H. canis

HPF-2(R)

GACTTCTCCTTCGTCTAAG

HEP-1(F)

CGCGAAATTACCCAATT^a

HEP-2(R)

CAGACCGGTTACTTTYAGCAG^a

HEP-4(R)

TAAGGTGCTGAAGGAGTCGTTTAT^a

HPF-1(F)

CTATGCCGACTAGAGATTG^a

18S rRNA

Piroplasmorida

BAB2 143–167 (F)

CCGTGCTAATTGTAGGGCTAATACA

551

[41]

Babesia vogeli

BAB2 694–667 (R)

GCTTGAAACACTCTARTTTTCTCAAAG

16S rRNA

Anaplasmataceae

EHR 16SD (F)

GGTACCYACAGAAGAAGTCC

344

[43]

Ehrlichia canis

EHR 16SD (R)

TAGCACTCATCGTTTACAG