Comparison of immunohistochemical and qPCR methods from granulomatous dermatitis lesions for detection of leishmania in dogs living in endemic areas: a preliminary study

Table 2 Inflammatory infiltrate (graded histologically) and parasite load (graded by histologic, IHC and biomolecular techniques) in the skin samples analyzed

Skin sample ID	Severity of dermal inflammation	Pattern of dermal inflammation	Depth of dermal inflammation	cPCR	Grading of parasite load
Skin sample ID	Severity of dermal inflammation	Pattern of dermal inflammation	Depth of dermal inflammation	cPCR	H&E staining^a	IHC^a	qPCR^a
1	Moderate	Interstitial	Deep	Positive	++	++	+++
2	Severe	Nodular	Deep	Positive	+++	+++	+++
3	Severe	Interstitial	Mid dermis	Positive	++	+++	+++
4	Moderate	Band-like	Mid dermis	Positive	+++	+++	++
5	Severe	Nodular	Deep	Positive	++	+	+++
6	Moderate	Interstitial	Mid dermis	Positive	+	+	+++
7	Severe	Diffuse	Deep	Positive	±	+	++
8	Severe	Nodular	Deep	Positive	±	+	++
9	Moderate	Perivascular	Mid dermis	Positive	+/−	++	+++
10	Severe	Diffuse	Mid dermis	Positive	−	+	+++
11	Moderate	Interstitial	Mid dermis	Positive	−	+/−	+++
12	Moderate	Nodular	Mid dermis	Positive	−	−	+
13	Severe	Diffuse	Deep	Positive	−	+	−
14	Mild	Perivascular	Superficial	Negative	+++	+++	+++
15	Moderate	Interstitial	Mid dermis	Negative	+++	++	+
16	Severe	Nodular	Deep	Negative	−	+	++
17	Moderate	Band-like	Deep	Negative	+/−	−	+ +
18	Moderate	Band-like	Superficial	Negative	−	−	+
19	Mild	Perivascular	Superficial	Negative	−	−	+
20	Mild	Perivascular	Mid dermis	Negative	−	−	+
21	Mild	Interstitial	Mid dermis	Negative	−	−	+
22	Moderate	Interstitial	Mid dermis	Negative	−	−	+
23	Severe	Nodular	Deep	Positive	−	−	−
24	Severe	Nodular	Deep	Negative	+/−	−	−
25	Severe	Diffuse	Deep	Negative	−	−	−
26	Mild	Perivascular	Superficial	Negative	−	−	−
27	Moderate	Noduslar	Deep	Negative	−	−	−
28	Moderate	n.a.	n.a	Negative	−	−	−
29	Mild	Perivascular	Superficial	Negative	−	−	−
30	Moderate	Perivascular	Mid dermis	Negative	−	−	−

ISSN: 1756-3305