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Table 2 Inflammatory infiltrate (graded histologically) and parasite load (graded by histologic, IHC and biomolecular techniques) in the skin samples analyzed

From: Comparison of immunohistochemical and qPCR methods from granulomatous dermatitis lesions for detection of leishmania in dogs living in endemic areas: a preliminary study

Skin sample ID

Severity of dermal inflammation

Pattern of dermal inflammation

Depth of dermal inflammation

cPCR

Grading of parasite load

H&E staininga

IHCa

qPCRa

1

Moderate

Interstitial

Deep

Positive

 ++ 

 ++ 

 +++ 

2

Severe

Nodular

Deep

Positive

 +++ 

 +++ 

 +++ 

3

Severe

Interstitial

Mid dermis

Positive

 ++ 

 +++ 

 +++ 

4

Moderate

Band-like

Mid dermis

Positive

 +++ 

 +++ 

 ++ 

5

Severe

Nodular

Deep

Positive

 ++ 

 + 

 +++ 

6

Moderate

Interstitial

Mid dermis

Positive

 + 

 + 

 +++ 

7

Severe

Diffuse

Deep

Positive

 ± 

 + 

 ++ 

8

Severe

Nodular

Deep

Positive

 ± 

 + 

 ++ 

9

Moderate

Perivascular

Mid dermis

Positive

 +/− 

 ++ 

 +++ 

10

Severe

Diffuse

Mid dermis

Positive

−

 + 

 +++ 

11

Moderate

Interstitial

Mid dermis

Positive

−

 +/−

 +++ 

12

Moderate

Nodular

Mid dermis

Positive

−

−

 + 

13

Severe

Diffuse

Deep

Positive

−

 + 

−

14

Mild

Perivascular

Superficial

Negative

 +++ 

 +++ 

 +++ 

15

Moderate

Interstitial

Mid dermis

Negative

 +++ 

 ++ 

 + 

16

Severe

Nodular

Deep

Negative

−

 + 

 ++ 

17

Moderate

Band-like

Deep

Negative

 +/− 

−

 + + 

18

Moderate

Band-like

Superficial

Negative

−

−

 + 

19

Mild

Perivascular

Superficial

Negative

−

−

 + 

20

Mild

Perivascular

Mid dermis

Negative

−

−

 + 

21

Mild

Interstitial

Mid dermis

Negative

−

−

 + 

22

Moderate

Interstitial

Mid dermis

Negative

−

−

 + 

23

Severe

Nodular

Deep

Positive

−

−

−

24

Severe

Nodular

Deep

Negative

 +/− 

−

−

25

Severe

Diffuse

Deep

Negative

−

−

−

26

Mild

Perivascular

Superficial

Negative

−

−

−

27

Moderate

Noduslar

Deep

Negative

−

−

−

28

Moderate

n.a.

n.a

Negative

−

−

−

29

Mild

Perivascular

Superficial

Negative

−

−

−

30

Moderate

Perivascular

Mid dermis

Negative

−

−

−

  1. n.a., Not applicable
  2. aSee Methods section for a description of the grading system applied