Skip to main content

Table 2 Inflammatory infiltrate (graded histologically) and parasite load (graded by histologic, IHC and biomolecular techniques) in the skin samples analyzed

From: Comparison of immunohistochemical and qPCR methods from granulomatous dermatitis lesions for detection of leishmania in dogs living in endemic areas: a preliminary study

Skin sample ID Severity of dermal inflammation Pattern of dermal inflammation Depth of dermal inflammation cPCR Grading of parasite load
H&E staininga IHCa qPCRa
1 Moderate Interstitial Deep Positive  ++   ++   +++ 
2 Severe Nodular Deep Positive  +++   +++   +++ 
3 Severe Interstitial Mid dermis Positive  ++   +++   +++ 
4 Moderate Band-like Mid dermis Positive  +++   +++   ++ 
5 Severe Nodular Deep Positive  ++   +   +++ 
6 Moderate Interstitial Mid dermis Positive  +   +   +++ 
7 Severe Diffuse Deep Positive  ±   +   ++ 
8 Severe Nodular Deep Positive  ±   +   ++ 
9 Moderate Perivascular Mid dermis Positive  +/−   ++   +++ 
10 Severe Diffuse Mid dermis Positive  +   +++ 
11 Moderate Interstitial Mid dermis Positive  +/−  +++ 
12 Moderate Nodular Mid dermis Positive  + 
13 Severe Diffuse Deep Positive  + 
14 Mild Perivascular Superficial Negative  +++   +++   +++ 
15 Moderate Interstitial Mid dermis Negative  +++   ++   + 
16 Severe Nodular Deep Negative  +   ++ 
17 Moderate Band-like Deep Negative  +/−   + + 
18 Moderate Band-like Superficial Negative  + 
19 Mild Perivascular Superficial Negative  + 
20 Mild Perivascular Mid dermis Negative  + 
21 Mild Interstitial Mid dermis Negative  + 
22 Moderate Interstitial Mid dermis Negative  + 
23 Severe Nodular Deep Positive
24 Severe Nodular Deep Negative  +/− 
25 Severe Diffuse Deep Negative
26 Mild Perivascular Superficial Negative
27 Moderate Noduslar Deep Negative
28 Moderate n.a. n.a Negative
29 Mild Perivascular Superficial Negative
30 Moderate Perivascular Mid dermis Negative
  1. n.a., Not applicable
  2. aSee Methods section for a description of the grading system applied