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Table 3 Cross-comparison and agreement of paired tests (H&E, IHC, cPCR and qPCR)

From: Comparison of immunohistochemical and qPCR methods from granulomatous dermatitis lesions for detection of leishmania in dogs living in endemic areas: a preliminary study

Diagnostic methods

Diagnostic method

N (%)

χ2 (p)/Overall agreement (k)

H&E staining [a]

IHC [b]

cPCR [c]

qPCR [d]

H&E staining [a]

8 (26.66%)

ne/ne

–

–

–

IHC [b]

14 (46.66%)

[a] vs [b]: 2.58(0.1)/ 0.8 (0.58)

ne/ne

 

–

cPCR [c]

14 (46.66%) 

[a] vs [c]: 2.58 (0.1)/ 0.66 (0.32)

[b] vs [c]: 0 (1)/ 0.8(0.65)

ne/ne

–

qPCR [d]

21 (70%)

[a] vs [d]:11.28 (< 0.001*)/ 0.56 (0.27)

[b] vs [d]: 1.76 (0.18)/ 0.7 (0.41)

[c] vs [d]: 1.76 (0.18) / 0.63 (0.28)

sne/ne

  1. n = 30 skin samples
  2. Îș, Kappa coefficient, ;  ne, not estimated
  3. *Statistical difference between methods for the same sample
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Parasites & Vectors

ISSN: 1756-3305

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