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Fig. 2a–c | Parasites & Vectors

Fig. 2a–c

From: The identification of alternative oxidase in intermediate host snails of Schistosoma and its potential role in protecting Oncomelania hupensis against niclosamide-induced stress

Fig. 2a–c

Dynamic expression profiles of Oncomelania hupensis AOX (OhAOX) messenger RNA (mRNA) and OhAOX protein in O. hupensis snails under wettable powder of niclosamide (WPN) stress. a Expression profiles of OhAOX in the whole snail, head and foot muscle (head-foot) region, and liver and gonad (liver-gonad) region after WPN treatment. Asterisks indicate significant difference (*P < 0.05, **P < 0.01) between time points and 0 h within the same treatment; underlined asterisks indicate significant difference between WPN- and H2O-treated snails at the same time point. b Expression of OhAOX protein (upper panel) in the whole snail 0–48 h after WPN and H2O treatment. The OhAOX mRNA profile calculated using quantitative real-time PCR (qPCR) is shown in the lower panel (and corresponds with the whole snail data in a). c Statistical analysis of OhAOX protein expression in the western blot (b); *P < 0.05, ***P < 0.001. bp Base pairs

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