Table 1 Germination of microsporidia for different electroporation parameters
From: Stable transformation of fluorescent proteins into Nosema bombycis by electroporation
Germination | Parameter | |||||
|---|---|---|---|---|---|---|
4 kV 200 Ω 25 µF | 3.5 kV 200 Ω 25 µF | 3 kV 200 Ω 25 µF | 2.5 kV 200 Ω 25 µF | 2 kV 200 Ω 25 µF | 1 kV 200 Ω 25 µF | |
0.1Â M KOH/10Â min | 22% | 15% | 10% | 30% | 25% | 21% |
0.1Â M KOH/20Â min | 23% | 17% | 10% | 29% | 25% | 25% |
0.1Â M KOH/30Â min | 20% | 17% | 11% | 32% | 28% | 22% |
0.1Â M DTT, 0.1Â M sorbitol, 0.1Â M SDS/10Â min | 25% | 20% | 10% | 30% | 28% | 25% |
0.5Â M DTT, 0.5Â M sorbitol, 0.5Â M SDS/10Â min | 27% | 20% | 15% | 32% | 29% | 28% |
1Â M DTT, 1Â M sorbitol, 1Â M SDS/10Â min | 25% | 22% | 11% | 35% | 32% | 30% |