Identification of severe fever with thrombocytopenia syndrome virus genotypes in patients and ticks in Liaoning Province, China

Han, Xiao-Hu; Ma, Yue; Liu, Hong-Yan; Li, Dan; Wang, Yan; Jiang, Feng-Hua; Gao, Qing-Tian; Jiang, Feng; Liu, Bao-Shan; Shen, Guo-Shun; Chen, Ze-Liang

doi:10.1186/s13071-022-05237-3

Table 1 SFTSV genome sequencing primer list

From: Identification of severe fever with thrombocytopenia syndrome virus genotypes in patients and ticks in Liaoning Province, China

Primer name	Primer sequence 5′ → 3′	Length (bp)	Tm (°C)	Start-stop	Amplicon (bp)
SF1	TACACAAAGACCCCCTTCATTTGG	24	61.29	1–24	749
SR1	GCTCATCATCCTCATCCAAGACAC	24	61.28	727–750	749
SF2	CTCCTCCAGATAGAGTCACTTGCA	24	61.41	582–605	646
SR2	TGAAGCCACAACCAAGACCCT	21	61.88	1207–1227	646
SF3	TAAACTTCTGTCTTGCTGGCTCC	23	60.81	1123–1145	623
SR3	ACACAAAGAACCCCAAAAAAGG	23	57.65	1724–1745	623
MF1	ACACAGTAGACGGCCAACAATGAT	24	62.61	1–24	587
MR1	TCTCCTCAGGGATGGGTGTCA	21	62.04	567–587	587
MF2	GATAGTTCCTGGGCCTTCATACAA	24	60.14	370–393	640
MR2	GAAACCRTAGCACTTTGGTCTGA	23	60.50	987–1009	640
MF3	GAGGCATCTGAGGCCAAGTG	20	60.75	907–926	677
MR3	CCTATTTCCTCATGGATCACTTGC	24	59.48	1560–1583	677
MF4	TGGGGRTCATGGGTCATAGCTC	22	61.36	1456–1477	586
MR4	CTGCCCAATCATCAGAAAAGG	22	61.36	2021–2041	586
MF5	GGAGTCCGGACTCAAAATGTC	21	58.65	1850–1870	685
MR5	GCGTCATCCACYCGTAGCTC	20	60.59	2515–2534	685
MF6	TTAGCTCCCTGCAACCAGGC	20	62.48	2386–2405	634
MR6	CCACAAATTTTGGGACATCCAG	22	58.07	2998–3019	634
MF7	GGGATGAGACTGCATTCAGTG	21	58.71	2852–2872	527
MR7	ACACAAAGACCGGCCAACACTTC	21	58.71	3356–3378	527
LF1	CGCCCAGATGGACTTG	16	54.13	10–25	414
LR1	CCAATGTTATGGCTCCTA	18	50.76	406–423	414
LF2	CCATTGTGGTGGTTGAA	17	51.82	378–394	607
LR2	TTGCTTGTTGCTYCTTG	19	57.12	966–984	607
LF3	GGCTATTGACAAAACTCA	16	54.13	10–25	414
LR3	AACCACATTACTGCTTAGRT	18	50.76	406–423	414
LF4	TCTTGTTGGAAAAGGCATC	19	53.43	1470–1488	608
LR4	GCATCTTTTGGGGCTTAG	20	59.26	2058–2077	608
LF5	GATGGAGGGCTTTGTCTC	18	54.67	2026–2043	645
LR5	TTTCTCAATCAATGTGGC	20	54.93	2651–2670	645
LF6	AGTACCATAGGTCCAAACTG	20	54.06	2619–2638	557
LR6	TCATTGCTTCCCTAAACG	20	56.50	3156–3175	557
LF7	TCYAGGTCAWCTGATCCGTTTA	22	59.10	3140–3161	491
LR7	TTGGTGRGACGCTGCWAT	20	60.96	3611–3630	491
LF8	CACAGGCACTTGGTTAGA	18	54.08	3578–3595	638
LR8	CAGGCTGCTAGTYACACC	20	62.53	4196–4215	638
LF9	ACTACAGRGCTCTRGATG	18	49.16	3981–3998	557
LR9	CTGGCTCTTGGAACAAGTCTAT	24	62.15	4514–4537	557
LF10	GCACCTAACAGGRAAATT	18	52.01	4462–4479	621
LR10	AATGGAMCCACCCCTAC	19	57.41	5064–5082	621
LF11	GGACCATGACTTTATGCC	18	52.70	5025–5042	601
LR11	TCGATTGCTCCAGAGGT	19	58.79	5607–5625	601
LF12	GGCGACATCCTTAACCT	17	53.20	5504–5520	497
LR12	TTCCACCTCAGCAGACC	19	59.70	5982–6000	497
LF13	CTCAGAGGGRTCRCTTGA	18	52.40	5929–5946	440
LR13	ACAAAGACCGCCCAGAT	19	60.00	6350–6368	440

SFTS, Severe fever with thrombocytopenia syndrome; Tm, primer melting temperature

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ISSN: 1756-3305

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