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Table 3 Median values of serum and OT EU according to the degree of reactivity to sera ELISA

From: Detection of specific antibodies against Leishmania infantum in canine serum and oral transudate using an in-house ELISA

Classification of dogs (number of dogs) Median of serum EU (min–max)a Median of OT EU (min–max)a
Total of dogs (407) 17.7 (0–300) 14.9 (0–300)
Sex
 Female (209) 22.3 (0–300) 13.8 (0–300)
 Male (198) 15.9 (0–300) 15.8 (0–300)
Ageb
 Young (79) 11.0 (1.8–300)c 9.9 (0–250.5)f
 Adult (267) 22.3 (0–300)c 18.1 (0–300)f
Breed
 Purebred (190) 16.9 (0–300) 16.0 (0–300)
 Mixed breed (217) 18.2 (0–300) 13.6 (0–300)
Geographical location
 Asturias (47) 3.7 (0–7.4)d 8.6 (0.2–39.9)g
 Barcelona (110) 11.4 (2.7–300)d 12.0 (0.2–300)g
 Cádiz (54) 6.3 (0–300)d 4.1 (0–300)g
 Mallorca (94) 25.3 (3.2–300)d 14.7 (2.2–166.5)g
Clinical status
 Sick (41) 300.0 (39.3–300)e 111.7 (11.6–300)h
 Apparently healthy (366) 12.8 (0–300)e 12.9 (0–300)h
Serological status
 Negative (258) 7.0 (0–34.7) 9.7 (0–76.4)
 Low positive (83) 59.2 (35–142.9) 38.1 (0–166.5)
 Medium positive (40) 210.4 (150.4–291.8) 80.4 (0–300)
 High positive (26) 300.0 (300) 160.9 (28.5–300)
Total positives (149) 132.8 (35–300) 59.2 (0–300)
  1. EU ELISA units, OT oral transudate, max maximum, min minimum
  2. aSamples with a value of 300 EU may actually be higher as the spectrophotometer is only able to read up to 3 units of optical density
  3. bAge was not recorded in 61 dogs
  4. cMann–Whitney test: U = 12,389, n1 = 267, n2 = 79, P = 0.018
  5. dKruskal–Wallis H-test: χ2 = 99.2, df = 3, P < 0.0001
  6. eMann–Whitney test: U = 829, n1 = 366, n2 = 41, P < 0.0001
  7. fMann–Whitney test: U = 12,863, n1 = 267, n2 = 79, P = 0.003
  8. gKruskal–Wallis H-test: χ2 = 38.7, df = 3, P < 0.0001
  9. hMann–Whitney test: U = 1461, n1 = 366, n2 = 41, P < 0.0001