Fig. 4

SjFer0 dsRNA interference in vivo. a SjFer0 mRNA expression levels detected by RT-qPCR. Error bars: 95% confidence intervals, n = 4. Asterisks indicate a significant difference at *P < 0.05, ***P < 0.001 (t-test). b Worm body length measurements. Asterisks indicate a significant difference at ****P < 0.0001 (t-test, n > 30). c Schistosome stained with carmine alum was observed by fluorescence microscopy. The gonad was less stained and there was little hemozoin deposition in the SjFer0 dsRNA interference group. Scale bar: 100 um. d Schistosome stained with carmine alum observed by laser scanning confocal microscopy (LSCM). The SjFer0 dsRNA interfered worm has a fewer eggs and less yolk than the GFP group. Scale bar: 25 um. Abbreviations: F, Female; GFP, green fluorescent protein; M, male; RT–qPCR, reverse transcription–quantitative PCR