Fig. 4

Egg laying differs among Ae. aegypti females that blood-fed once (a) or had ad libitum access to BSA- (b), HGB- (c) or tryptone-sucrose (d). Newly eclosed females were individually housed in small cages with oviposition substrates replaced daily until death. Females were blood-fed 4 days post-eclosion while protein-sucrose solutions were provided on day 1 post-emergence. Circles indicate the number of eggs laid by an individual female: black circles indicate the female laid eggs only once while gray circles indicate females that laid eggs more than once. Numbers inside each gray circle indicate the female while the y axis indicates the number of eggs laid on a given day. (e) Kaplan-Meier plot showing the survival of the same females used to assess egg laying in a–d. Total number of females monitored for each treatment was 30 (blood), 32 (BSA-sucrose), 35 (HGB-sucrose) and 35 (tryptone-sucrose)