Fig. 8From: Ad libitum consumption of protein- or peptide-sucrose solutions stimulates egg formation by prolonging the vitellogenic phase of oogenesis in anautogenous mosquitoesRNAi knockdown of EcR, topical application of the JH analog methoprene and feeding of the TOR inhibitor rapamycin disable oocyte maturation when Ae. aegypti females are fed BSA-sucrose. a, b Females injected with dsEcR RNA exhibit a significant reduction in EcR transcript abundance and yolk deposition into oocytes compared to females injected with dsEGFP. c, d Females topically treated with methoprene (250 ng) in ethanol exhibit a significant increase in transcript abundance of the JH-responsive gene Hairy but reduced yolk deposition into oocytes compared to females topically treated with ethanol (EtOH) alone. e Females that consume BSA-sucrose containing rapamycin dissolved in DMSO deposit significantly lower amounts of yolk into oocytes than females that consume BSA-sucrose containing an equivalent amount of DMSO alone. Yolk length was measured in individual treatment and control females as described in Fig. 1Back to article page