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Fig. 8 | Parasites & Vectors

Fig. 8

From: Ad libitum consumption of protein- or peptide-sucrose solutions stimulates egg formation by prolonging the vitellogenic phase of oogenesis in anautogenous mosquitoes

Fig. 8

RNAi knockdown of EcR, topical application of the JH analog methoprene and feeding of the TOR inhibitor rapamycin disable oocyte maturation when Ae. aegypti females are fed BSA-sucrose. a, b Females injected with dsEcR RNA exhibit a significant reduction in EcR transcript abundance and yolk deposition into oocytes compared to females injected with dsEGFP. c, d Females topically treated with methoprene (250 ng) in ethanol exhibit a significant increase in transcript abundance of the JH-responsive gene Hairy but reduced yolk deposition into oocytes compared to females topically treated with ethanol (EtOH) alone. e Females that consume BSA-sucrose containing rapamycin dissolved in DMSO deposit significantly lower amounts of yolk into oocytes than females that consume BSA-sucrose containing an equivalent amount of DMSO alone. Yolk length was measured in individual treatment and control females as described in Fig. 1

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