Fig. 9

Females provided ad libitum access to BSA- or tryptone-sucrose exhibit a persistent reduction in attraction to a human hand. a Newly eclosed Ae. aegypti females (F0) were tested for attraction to a human hand daily for 3 weeks when fed water only, 10% sucrose, blood, BSA-sucrose or tryptone-sucrose. b Aedes aegypti females maintained for five generations (F5) on BSA-sucrose exclusively were similarly tested for attraction for the same treatments as in (a). For each treatment, five replicate cages containing ten females each were used for the assay. In the left panel, dots indicate the mean proportion of females that flew to the top of the cage and began probing within 1 min of hand presentation; error bars (gray) indicate standard error. Oviposition substrates were offered when females were 4 days old, and day of egg laying by protein-fed females is indicated. In the right panel, graphs present the same daily means (dots) but overlaid with color-coded slopes indicating linear regression analysis of the mean proportion of attracted females over time, with shaded areas bound by dotted lines indicating upper and lower 95% confidence intervals