Table 2 Sequencing statistics for each sample type. Calculation of generated data and coverage was not possible for sample GER because of significant carry-over from a previous run on the same flow cell
From: A barcoding pipeline for mosquito surveillance in Nepal, a biodiverse dengue-endemic country
Sample | Run time (h) | Reads generated | Data retrieved after basecall (.fastq) | Coverage after demultiplexing per sample | Variance in coverage between single samples |
|---|---|---|---|---|---|
NP2 | 4:00 | 2.57 M | 4.0 GB | 37,578 × | 4151–135,874 × |
NP1 | 3:45 | 1.53 M | 2.5 GB | 3965 × | 6–13,307 × |
BEL | 3:45 | 34,302 × | 416–97,623 × | ||
GER | 5:00 | NA | NA | NA | NA |