Table 3 Overview of identified species (NP1) using either classical morphological identification or ONS followed by a BLAST against the GenBank database or by a BOLD search
From: A barcoding pipeline for mosquito surveillance in Nepal, a biodiverse dengue-endemic country
Sample | Species (morphological identification) | Species (ONS sequence) | Percent ident. (BLAST) | Matching of results | Accession number (ONS) | Reference for identification of sequencing result |
|---|---|---|---|---|---|---|
NP1-1 | Ae. albopictus | Ae. albopictus | 98.6 | Y | OL352190 | Batovska et al. [49] |
NP1-2 | An. splendidus | An. subpictus/jamesii | 98.5/98.3 | N | OL352191 | Â |
NP1-3 | An. annularis | An. annularis | 98.8 | Y | OL352192 | Ashfaq et al. [45] |
NP1-4 | An. barbirostris | An. barbirostris | 99.9 | Y | OL352193 | Saeung et al. [47] |
NP1-5 | An. culicifacies | An. culicifacies | 98.8 | Y | OL352194 | Ashfaq et al. [45] |
NP1-6 | An. maculatus | An. dravidicus | 99.4 | N | OL352195 | Ashfaq et al. [45] |
NP1-7 | An. nivipes | An. annularis | 98.9 | N | OL352196 | Ashfaq et al. [45] |
NP1-8 | An. sinensis | An. nivipes | 99.5 | N | OL352197 | Â |
NP1-9 | An. athakani | An. lindesayi | 99.5 | N | OL352198 | Namgay et al. [46] |
NP1-10 | An. culicifacies | An. culicifacies | 98.9 | Y | OL352199 | Ashfaq et al. [45] |
NP1-11 | An. dravidicus | An. maculatus | 93.1 | N | OL352200 | Â |
NP1-12 | An. fluviatilis | An. aconitus | 97.4 | N | OL352201 | Wilkerson et al. [48] |
NP1-13 | An. nigerrimus | An. peditaeniatus | 99.7 | N | OL352202 | Ashfaq et al. [45] |
NP1-14 | An. splendidus | An. subpictus/jamesii | 99.1/99.5 | N | OL352203 | Â |
NP1-15 | An. tessellatus | An. tessellatus | 97.7 | Y | OL352204 | Bourke et al. [44] |