Fig. 2

Differential gene expression analysis between mouthpart (MP) and bloodstream form (BSF) T. congolense parasites. a Read count density expressed as log-counts per million reads mapped (log₂CPM) per sample before and after filtering transcripts with less than 10 read counts. b Distribution of log₂CPM values per sample before and after normalisation for library size. c Multidimensional scaling plot showing the distances between gene expression profiles of each experimental group. d Volcano plot showing change in expression between conditions and transcript abundance, expressed as log₂ fold change and log₂cpm. Differentially expressed genes (log2FC >|1.5| and P-value < 0.01) are highlighted in blue (downregulated) or red (upregulated in mouthpart parasites)