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Fig. 1 | Parasites & Vectors

Fig. 1

From: Ivermectin-induced gene expression changes in adult Parascaris univalens and Caenorhabditis elegans: a comparative approach to study anthelminthic metabolism and resistance in vitro

Fig. 1

Illustration of the study design. Section a depicts experimental setup of Parascaris univalens. Adult worms were exposed to IVM drug concentrations, 10–13, 10–11, 10–9 M, and media+DMSO (control). After viability scoring, RNA was sequenced, candidate genes selected, and quantitative RT-qPCR performed on the selected candidate genes. Section b depicts experimental setup of Caenorhabditis elegans. Worm pools of adults at maximum reproduction were exposed to IVM drug concentrations, 10–9, 10–8, 10–7 M, and media+DMSO (control). Quantitative RT-PCR was performed on the P. univalens orthologues of selected candidate genes. Relative gene expression of the candidates was compared between nematode species

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